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pact 2 vector  (TaKaRa)


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    Structured Review

    TaKaRa pact 2 vector
    Interaction of PPP1R2P3 with different PPP1 isoforms. ( A ), Sequential transformation of yeast AH109 with bait plasmid (pAS2-PPP1CA, pAS2-PPP1CC1, pAS2-PPP1CC2, or pAS2-PPP1CC2end) and the prey plasmid pACT2-PPP1R2P3. pAS2-PPP1CC2end is the unique C-terminal tail of PPP1CC2 produced by alternative splicing of the PPP1CC gene. For negative and positive controls <t>pAS2-1/pACT-2</t> and pVA3-1/pTD1-1 vectors were used, respectively ( B ), Overlay assay of PPP1R2 and PPP1R2P3. Commercial PPP1R2 and His-PPP1R2P3 were separated by SDS-PAGE, transferred to nitrocellulose and overlaid with recombinant PPP1CC1 or PPP1CC2, as indicated. Western blotting was performed with the respective specific antibodies.
    Pact 2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pact+2/pmCherry+Vector/pmc03606321-218-13-15
    Average 96 stars, based on 619 article reviews
    pact 2 vector - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa"

    Article Title: Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa

    Journal: BMC Cell Biology

    doi: 10.1186/1471-2121-14-15

    Interaction of PPP1R2P3 with different PPP1 isoforms. ( A ), Sequential transformation of yeast AH109 with bait plasmid (pAS2-PPP1CA, pAS2-PPP1CC1, pAS2-PPP1CC2, or pAS2-PPP1CC2end) and the prey plasmid pACT2-PPP1R2P3. pAS2-PPP1CC2end is the unique C-terminal tail of PPP1CC2 produced by alternative splicing of the PPP1CC gene. For negative and positive controls pAS2-1/pACT-2 and pVA3-1/pTD1-1 vectors were used, respectively ( B ), Overlay assay of PPP1R2 and PPP1R2P3. Commercial PPP1R2 and His-PPP1R2P3 were separated by SDS-PAGE, transferred to nitrocellulose and overlaid with recombinant PPP1CC1 or PPP1CC2, as indicated. Western blotting was performed with the respective specific antibodies.
    Figure Legend Snippet: Interaction of PPP1R2P3 with different PPP1 isoforms. ( A ), Sequential transformation of yeast AH109 with bait plasmid (pAS2-PPP1CA, pAS2-PPP1CC1, pAS2-PPP1CC2, or pAS2-PPP1CC2end) and the prey plasmid pACT2-PPP1R2P3. pAS2-PPP1CC2end is the unique C-terminal tail of PPP1CC2 produced by alternative splicing of the PPP1CC gene. For negative and positive controls pAS2-1/pACT-2 and pVA3-1/pTD1-1 vectors were used, respectively ( B ), Overlay assay of PPP1R2 and PPP1R2P3. Commercial PPP1R2 and His-PPP1R2P3 were separated by SDS-PAGE, transferred to nitrocellulose and overlaid with recombinant PPP1CC1 or PPP1CC2, as indicated. Western blotting was performed with the respective specific antibodies.

    Techniques Used: Transformation Assay, Plasmid Preparation, Produced, Overlay Assay, SDS Page, Recombinant, Western Blot

    Related Articles

    cDNA Library Assay:

    Article Title: Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system
    Article Snippet: .. The commercially available human testis cDNA library, cloned into pACT-2, a GAL-4 activation domain vector, and transformed into Y-187 yeast strain, was purchased from Clontech Laboratories and used for screening in the Y2H system. .. The library was tested for the expression of proteins using the murine monoclonal antibody to the activation domain (aa 768–881) (Clontech Laboratories) by the Western blot procedure.

    Article Title: Interaction of the human somatostatin receptor 3 with the multiple PDZ domain protein MUPP1 enables somatostatin to control permeability of epithelial tight junctions.
    Article Snippet: a Institut für Zellbiochemie und klinische Neurobiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany b Institut für Humangenetik, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany c Institut für Angewandte Physiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany d Klinik und Poliklinik für Dermatologie und Venerologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany e Computational Biology Programme, EMBL, 69117 Heidelberg, Germany f Institut für Pharmakologie und Toxikologie, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany

    Article Title: AIRE recruits multiple transcriptional components to specific genomic regions through tethering to nuclear matrix.
    Article Snippet: 0 d Thymic selection requires that diverse self antigens be presented to developing thymocytes by stromal cells.. Consistent with this function, edullary thymic epithelial cells have been shown to express a large number of genes, many of which are tissue restricted.. Autoimmune egulator (AIRE) is a nuclear protein, which has recently been identified as a regulator of this process, however, the mechanism by which IRE functions is not well understood.

    Article Title: Postsynaptic Shank Antagonizes Dendrite Branching Induced by the Leucine-Rich Repeat Protein Densin-180
    Article Snippet: The cDNA coding for the Src homology 3 (SH3) domain of human somatostatin receptor interacting protein (SSTRIP)/ Shank1 (amino acids 444 – 646) (Zitzer et al., 1999) was amplified by PCR and subcloned into the pAS2 vector (Clontech, Heidelberg, Germany). .. This bait plasmid was cotransformed together with a pretransformed human brain Matchmaker cDNA library in pACT-2 (Clontech) into the CG1945 yeast strain. ..

    Article Title: Postsynaptic Shank Antagonizes Dendrite Branching Induced by the Leucine-Rich Repeat Protein Densin-180
    Article Snippet: The cDNA coding for the Src homology 3 (SH3) domain of human somatostatin receptor interacting protein (SSTRIP)/Shank1 (amino acids 444-646) ( Zitzer et al., 1999 ) was amplified by PCR and subcloned into the pAS2 vector (Clontech, Heidelberg, Germany). .. This bait plasmid was cotransformed together with a pretransformed human brain Matchmaker cDNA library in pACT-2 (Clontech) into the CG1945 yeast strain. ..

    Clone Assay:

    Article Title: Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system
    Article Snippet: .. The commercially available human testis cDNA library, cloned into pACT-2, a GAL-4 activation domain vector, and transformed into Y-187 yeast strain, was purchased from Clontech Laboratories and used for screening in the Y2H system. .. The library was tested for the expression of proteins using the murine monoclonal antibody to the activation domain (aa 768–881) (Clontech Laboratories) by the Western blot procedure.

    Article Title: Antibodies against gene products related to Werner's Syndrome
    Article Snippet: .. A library of 1.1×106 independent cDNA clones generated from RNA isolated from stimulated human peripheral blood mononuclear cells is generated in pACT-2 (Clontech, Palo Alto, Calif.) that creates cDNA/GAL4 activation domain fusions is co-transfected into yeast containing pLEXA with the WRN gene fragment to generate WRN/LEXA DNA-binding fusion. ..

    Article Title: JLP Associates with Kinesin Light Chain 1 through a Novel Leucine Zipper-like Domain
    Article Snippet: .. The PCR product was subsequently cloned into pACT-2 (Clontech) via its EcoR1 and Xho sites. ..

    Article Title: AIRE recruits multiple transcriptional components to specific genomic regions through tethering to nuclear matrix.
    Article Snippet: 0 d Thymic selection requires that diverse self antigens be presented to developing thymocytes by stromal cells.. Consistent with this function, edullary thymic epithelial cells have been shown to express a large number of genes, many of which are tissue restricted.. Autoimmune egulator (AIRE) is a nuclear protein, which has recently been identified as a regulator of this process, however, the mechanism by which IRE functions is not well understood.

    Activation Assay:

    Article Title: Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system
    Article Snippet: .. The commercially available human testis cDNA library, cloned into pACT-2, a GAL-4 activation domain vector, and transformed into Y-187 yeast strain, was purchased from Clontech Laboratories and used for screening in the Y2H system. .. The library was tested for the expression of proteins using the murine monoclonal antibody to the activation domain (aa 768–881) (Clontech Laboratories) by the Western blot procedure.

    Article Title: Antibodies against gene products related to Werner's Syndrome
    Article Snippet: .. A library of 1.1×106 independent cDNA clones generated from RNA isolated from stimulated human peripheral blood mononuclear cells is generated in pACT-2 (Clontech, Palo Alto, Calif.) that creates cDNA/GAL4 activation domain fusions is co-transfected into yeast containing pLEXA with the WRN gene fragment to generate WRN/LEXA DNA-binding fusion. ..

    Transformation Assay:

    Article Title: Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system
    Article Snippet: .. The commercially available human testis cDNA library, cloned into pACT-2, a GAL-4 activation domain vector, and transformed into Y-187 yeast strain, was purchased from Clontech Laboratories and used for screening in the Y2H system. .. The library was tested for the expression of proteins using the murine monoclonal antibody to the activation domain (aa 768–881) (Clontech Laboratories) by the Western blot procedure.

    Article Title: Functional specialization of duplicated AP3-like genes in Medicago truncatula.
    Article Snippet: Edel ın Roque, Joanna Serwatowska, M. Cruz Rochina, Jiangqi Wen, Kirankumar S. Mysore, Lynne Yenush, Jos e P ıo Beltr an and Luis A. Ca~ nas* Ciudad Polit ecnica de la Innovaci on, Instituto de Biolog ıa Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientı́ficas-Universidad Politécnica de Valencia, Edificio 8E, Calle Ingeniero Fausto Elio s/n, E–46011 Valencia, Spain, and Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore OK 73401, USA

    Generated:

    Article Title: Antibodies against gene products related to Werner's Syndrome
    Article Snippet: .. A library of 1.1×106 independent cDNA clones generated from RNA isolated from stimulated human peripheral blood mononuclear cells is generated in pACT-2 (Clontech, Palo Alto, Calif.) that creates cDNA/GAL4 activation domain fusions is co-transfected into yeast containing pLEXA with the WRN gene fragment to generate WRN/LEXA DNA-binding fusion. ..

    Isolation:

    Article Title: Antibodies against gene products related to Werner's Syndrome
    Article Snippet: .. A library of 1.1×106 independent cDNA clones generated from RNA isolated from stimulated human peripheral blood mononuclear cells is generated in pACT-2 (Clontech, Palo Alto, Calif.) that creates cDNA/GAL4 activation domain fusions is co-transfected into yeast containing pLEXA with the WRN gene fragment to generate WRN/LEXA DNA-binding fusion. ..

    Polymerase Chain Reaction:

    Article Title: JLP Associates with Kinesin Light Chain 1 through a Novel Leucine Zipper-like Domain
    Article Snippet: .. The PCR product was subsequently cloned into pACT-2 (Clontech) via its EcoR1 and Xho sites. ..

    Derivative Assay:

    Article Title: Interaction of the human somatostatin receptor 3 with the multiple PDZ domain protein MUPP1 enables somatostatin to control permeability of epithelial tight junctions.
    Article Snippet: a Institut für Zellbiochemie und klinische Neurobiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany b Institut für Humangenetik, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany c Institut für Angewandte Physiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany d Klinik und Poliklinik für Dermatologie und Venerologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany e Computational Biology Programme, EMBL, 69117 Heidelberg, Germany f Institut für Pharmakologie und Toxikologie, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany

    Plasmid Preparation:

    Article Title: Interaction of the human somatostatin receptor 3 with the multiple PDZ domain protein MUPP1 enables somatostatin to control permeability of epithelial tight junctions.
    Article Snippet: a Institut für Zellbiochemie und klinische Neurobiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany b Institut für Humangenetik, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany c Institut für Angewandte Physiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany d Klinik und Poliklinik für Dermatologie und Venerologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany e Computational Biology Programme, EMBL, 69117 Heidelberg, Germany f Institut für Pharmakologie und Toxikologie, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany

    Article Title: Postsynaptic Shank Antagonizes Dendrite Branching Induced by the Leucine-Rich Repeat Protein Densin-180
    Article Snippet: The cDNA coding for the Src homology 3 (SH3) domain of human somatostatin receptor interacting protein (SSTRIP)/ Shank1 (amino acids 444 – 646) (Zitzer et al., 1999) was amplified by PCR and subcloned into the pAS2 vector (Clontech, Heidelberg, Germany). .. This bait plasmid was cotransformed together with a pretransformed human brain Matchmaker cDNA library in pACT-2 (Clontech) into the CG1945 yeast strain. ..

    Article Title: Postsynaptic Shank Antagonizes Dendrite Branching Induced by the Leucine-Rich Repeat Protein Densin-180
    Article Snippet: The cDNA coding for the Src homology 3 (SH3) domain of human somatostatin receptor interacting protein (SSTRIP)/Shank1 (amino acids 444-646) ( Zitzer et al., 1999 ) was amplified by PCR and subcloned into the pAS2 vector (Clontech, Heidelberg, Germany). .. This bait plasmid was cotransformed together with a pretransformed human brain Matchmaker cDNA library in pACT-2 (Clontech) into the CG1945 yeast strain. ..



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    Image Search Results


    Interaction of PPP1R2P3 with different PPP1 isoforms. ( A ), Sequential transformation of yeast AH109 with bait plasmid (pAS2-PPP1CA, pAS2-PPP1CC1, pAS2-PPP1CC2, or pAS2-PPP1CC2end) and the prey plasmid pACT2-PPP1R2P3. pAS2-PPP1CC2end is the unique C-terminal tail of PPP1CC2 produced by alternative splicing of the PPP1CC gene. For negative and positive controls pAS2-1/pACT-2 and pVA3-1/pTD1-1 vectors were used, respectively ( B ), Overlay assay of PPP1R2 and PPP1R2P3. Commercial PPP1R2 and His-PPP1R2P3 were separated by SDS-PAGE, transferred to nitrocellulose and overlaid with recombinant PPP1CC1 or PPP1CC2, as indicated. Western blotting was performed with the respective specific antibodies.

    Journal: BMC Cell Biology

    Article Title: Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa

    doi: 10.1186/1471-2121-14-15

    Figure Lengend Snippet: Interaction of PPP1R2P3 with different PPP1 isoforms. ( A ), Sequential transformation of yeast AH109 with bait plasmid (pAS2-PPP1CA, pAS2-PPP1CC1, pAS2-PPP1CC2, or pAS2-PPP1CC2end) and the prey plasmid pACT2-PPP1R2P3. pAS2-PPP1CC2end is the unique C-terminal tail of PPP1CC2 produced by alternative splicing of the PPP1CC gene. For negative and positive controls pAS2-1/pACT-2 and pVA3-1/pTD1-1 vectors were used, respectively ( B ), Overlay assay of PPP1R2 and PPP1R2P3. Commercial PPP1R2 and His-PPP1R2P3 were separated by SDS-PAGE, transferred to nitrocellulose and overlaid with recombinant PPP1CC1 or PPP1CC2, as indicated. Western blotting was performed with the respective specific antibodies.

    Article Snippet: PPP1R2P3 cDNA was excised using EcoRI and XhoI from pET-PPP1R2P3 and subcloned in pACT-2 vector (Clontech, Saint Germain-en-Laye, France) for co-transformation.

    Techniques: Transformation Assay, Plasmid Preparation, Produced, Overlay Assay, SDS Page, Recombinant, Western Blot