pact 2 vector (TaKaRa)
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Pact 2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pact+2/pmCherry+Vector/pmc03606321-218-13-15
Average 96 stars, based on 619 article reviews
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1) Product Images from "Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa"
Article Title: Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa
Journal: BMC Cell Biology
doi: 10.1186/1471-2121-14-15
Figure Legend Snippet: Interaction of PPP1R2P3 with different PPP1 isoforms. ( A ), Sequential transformation of yeast AH109 with bait plasmid (pAS2-PPP1CA, pAS2-PPP1CC1, pAS2-PPP1CC2, or pAS2-PPP1CC2end) and the prey plasmid pACT2-PPP1R2P3. pAS2-PPP1CC2end is the unique C-terminal tail of PPP1CC2 produced by alternative splicing of the PPP1CC gene. For negative and positive controls pAS2-1/pACT-2 and pVA3-1/pTD1-1 vectors were used, respectively ( B ), Overlay assay of PPP1R2 and PPP1R2P3. Commercial PPP1R2 and His-PPP1R2P3 were separated by SDS-PAGE, transferred to nitrocellulose and overlaid with recombinant PPP1CC1 or PPP1CC2, as indicated. Western blotting was performed with the respective specific antibodies.
Techniques Used: Transformation Assay, Plasmid Preparation, Produced, Overlay Assay, SDS Page, Recombinant, Western Blot
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